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KMID : 0380919960250010163
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Journal of the Korean Society of Food Nutrition
1996 Volume.25 No. 1 p.163 ~ p.169
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Characterization of Chlorina Mutant Induced from the Nagdongbyeo(Oryza sativa L.)
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Kim Hong-Sub
Lim Chae-Kyu
Koh Jae-Moon
Kim Jong-Se
Lee Sook-Young
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Abstract
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Chlorina(ch) mutant, the chlorophyll-deficiency strain was induced from the original rice called Nagdongbyeo(Oryza sativa L.). The present study have been undertaken to characterize chlorina mutant by comparing with the original relating to physiological and biochemical properties. The chlorina mutant would be useful as a strain to detect genes involved with chlorophyll deficiency because of yellow color development in early stage of growth. a/b content ratio of chlorophyll in the ch mutant was identical with the original in all growth period but total chlorophyll contents were only 70.2% of the original. Anthocyanin and flavonol of ch mutant were remarkably increased to 285 and 142% respectively. Also the contents of carotenoids and xanthophylls in ch mutant were given 71.7 and 56.5% of the original respectively. In case of carotenoid lutein and neoxanthin decreased to 32 and 34.4%, respectively but antheranthins increased to 106.7%. β-Carotene content was the same as the original. In the individual or community condition, the reflexibility and transmission was increased in ch mutant than those in original but absortivity decreased. The earing season of ch mutant was five days late as compared to origianl ; in addition, culm and panicle length, number of panicle per hill and spikelets per panicle ripened grain ratio, 1,000-grain weight and yield decreased. Any significant difference was not observed in the pattern of isozyme such as esterase, phosphoglucose isomerase, malic enzyme and hexokinase. But the total protein pattern of chloroplast was remarkably different from each other.
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KEYWORD
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chlorina mutant, original Nagdongbyeo
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